In-silico characterization of VacA virulence factor of Helicobacter pylori

Authors

  • Nadia Hussain Department of Pharmaceutical Sciences, College of Pharmacy, Al Ain University, Al Ain Campus, 64141 Al Ain, United Arab Emirates. Author
  • Saboor Muarij Bunny Research Associate, Pakistan Biosafety Clearing House-Pakistan Environmental Protection Agency Author
  • Amal H. I. Al Haddad Chief Operations Office, Sheikh Shakhbout Medical City (SSMC), PureHealth, Abu Dhabi, UAE Author
  • Wardah Shahid Institute of Chemistry, The Islamia University of Bahawalpur, Pakistan Author
  • Shafia Arshad Office of Research Innovation and Commercialization, The Govt. Sadiq College Women University Bahawalpur, Pakistan Author
  • Fatima Muccee School of Biochemistry and Biotechnology, University of the Punjab, Lahore, Pakistan Author

DOI:

https://doi.org/10.63075/a8c68g38

Abstract

Helicobacter pylori is associated with incidence of gastric cancer due to several virulence factors. Its virulence can be reduced through inhibiting its toxins. Vacuolating cytotoxin (VacA) is one of its virulence factors. Current study focused at characterization of VacA. Its sequence was retrieved from Uniprot database and analyzed through SOPMA tool, ALPHAFOLD database, STRING tool and QuickGo. Analyses demonstrated that alpha helix, beta pleated sheets and random coil were composed of 17, 26.94 and 56.06% amino acids, respectively. The 3D configuration identified two major domains one significantly comprised of beta sheet and other of alpha helix. Protein interaction analysis revealed three protein partners with highest scores. i. e., ureB (0.733), hpaA (0.704) and dps (0.702). Gene ontology revealed its function in toxicity. These findings might help in genetic alteration of VacA in order to inhibit its involvement in gastric cancer.

 

Keywords: Helicobacter pylori, vacuolating cytotoxin A, gene ontology, configuration, interaction

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Published

2025-08-05

Issue

Vol. 3 No. 8 (2025): AMARR

Section

Applied Sciences

How to Cite

In-silico characterization of VacA virulence factor of Helicobacter pylori. (2025). Annual Methodological Archive Research Review, 3(8), 193-201. https://doi.org/10.63075/a8c68g38